Engineer-it Kit™: Refill

This Kit is a refill for our genetic engineering kit for bacteria, the Engineer-it Kit. It does not come with DNA or bacteria (cells). Use DNA and bacteria from a previous Engineer-it Kit, Smell-it Kit, Induce-it Kit, Blue-it Kit, or Heat-it Kit or use DNA you have created yourself. 

This science project refill kit can be used for: 

• completing more genetic engineering experiments with your existing bacteria and DNA program from the regular Engineer-it Kit, or any of the advanced kits,
• culturing cells that you have already engineered (e.g. you want to pick a colony and streak some engineered bacteria to grow more)

This refill kit is a perfect way to extend biotechnology experiments at home, in the classroom, the makerspace, community center, or in a local biohacking DIYbio lab!

A course on Udemy guides you through the Engineer-it Kit experience and provides confidence, context, and theory. 

Unsure what biotechnology and genetic engineering are? Have a look at our short"What is Biotechnology? Genetic Engineering?" post. And remember, no prior knowledge or skills are necessary to get started with Amino Labs beginner's biotechnology kits!  

During this biotechnology/genetic engineering experiment, you will learn how to use DNA programs (called plasmids) to make with biology.

The steps for this experiment are:
1. Make selective & non-selective agar,
2. pour Petri dishes,
3. streak bacteria using your own bacteria,
4. make bacteria chemically competent,
5. engineer bacteria using pigment-producing DNA plasmid (using your own DNA),
6. create positive and negative controls,
7. incubate,
8. view/analyze your results!

(1) Engineer-it Kit in REFILL Individual size: Sterile water, 6cm Petri dish, LB agar, selection marker, transformation buffer, recovery media, inactivation bag. Since this is a Refill kit, no DNA, bacteria or streaking stencil is included. Use the ones from a previous experiment!

• Individual Kits: An individual kit with the ingredients needed for completing one entire science experience. Great for an individual, family, or a small group — best results using a DNA Playground home or large.

• To pick which antibiotics you need from the drop-down menu:

• If you are using a DNA program and bacteria from the color-pigment Engineer-it kit, you will need the antibiotics Chlor, and no chemicals.
• If you are using a DNA program and bacteria from the Heat-it kit, you will need Chlor and no chemicals
• If you are using a DNA program and bacteria from the Smell-it kit, you will need Chlor and IAA chemical
• If you are using a DNA program and bacteria from the Blue-it kit, you will need Chlor and X-gal chemical
• If you are using a DNA program and bacteria from the Induce-it kit, you will need Amp and IPTG
• If you are using your own DNA program and bacteria, select accordingly


• Instructions: a manual with easy-to-understand vocabulary and step-by-step procedures and an online experiment simulation to practice with, teach you fundamentals, and guide you along the way. See the Resources section below for links

This kit is a refill and does not include any bacteria or DNA. Use the bacteria and DNA from an Engineer-it Kit or your own lab.

Cleaning and safety supplies are not included in the kit. Necessary safety and cleaning supplies are latex or nitrile gloves, and a chlorinated bleach cleaner or a 10% solution of chlorinated bleach in a spray bottle (you can make this by adding 9 parts water to 1 part bleach in a spray bottle). You can add a Mini Safety Set to your kit which includes gloves and a reusable plastic apron to protect your clothes.

For more information about safety, set up and clean up, you can have a look at the instruction manual for the experiment linked in the Ressources section below, and at the "Practicing safe science" page.

Shipping Times

Experiment kits are shipped 1-10 working days after you place your order. If you require yours for a specific date or event, please contact us before or with your order # so that we can try to make it happen for you! ☺︎ 

Storage

For the best results, and the longest shelf life possible, refrigerate the kit(s) upon arrival. You'll find a "Best by" date on the kit's label. This date assumes you've refrigerated your kit upon arrival.

For any questions, don't hesistate to reach out to us - contact us