Engineer-it Kit™: Refill

US$30.00
Antibiotic
Chemical

This Kit is a refill for our genetic engineering kit for bacteria, the Engineer-it Kit. It does not come with DNA or bacteria (cells). Use DNA and bacteria from a previous Engineer-it Kit, Smell-it Kit, Induce-it Kit, Blue-it Kit, or Heat-it Kit or use DNA you have created yourself. 

This science project refill kit can be used for: 

  • completing more genetic engineering experiments with your existing bacteria and DNA program from the regular Engineer-it Kit, or any of the advanced kits,
  • culturing cells that you have already engineered (e.g. you want to pick a colony and streak some engineered bacteria to grow more)

This refill kit is a perfect way to extend biotechnology experiments at home, in the classroom, the makerspace, community center, or in a local biohacking DIYbio lab!

A course on Udemy guides you through the Engineer-it Kit experience and provides confidence, context, and theory. 

Unsure what biotechnology and genetic engineering are? Have a look at our short"What is Biotechnology? Genetic Engineering?" post. And remember, no prior knowledge or skills are necessary to get started with Amino Labs beginner's biotechnology kits!  

 

During this biotechnology/genetic engineering experiment, you will learn how to use DNA programs (called plasmids) to make with biology.

The steps for this experiment are:
1. Make selective & non-selective agar,
2. pour Petri dishes,
3. streak bacteria using your own bacteria,
4. make bacteria chemically competent,
5. engineer bacteria using pigment-producing DNA plasmid (using your own DNA),
6. create positive and negative controls,
7. incubate,
8. view/analyze your results!

(1) Engineer-it Kit in REFILL Individual size: Sterile water, 6cm Petri dish, LB agar, selection marker, transformation buffer, recovery media, inactivation bag. Since this is a Refill kit, no DNA, bacteria or streaking stencil is included. Use the ones from a previous experiment!

• Individual Kits: An individual kit with the ingredients needed for completing one entire science experience. Great for an individual, family, or a small group — best results using a DNA Playground home or large.

• To pick which antibiotics you need from the drop-down menu:

• If you are using a DNA program and bacteria from the color-pigment Engineer-it kit, you will need the antibiotics Chlor, and no chemicals.
• If you are using a DNA program and bacteria from the Heat-it kit, you will need Chlor and no chemicals
• If you are using a DNA program and bacteria from the Smell-it kit, you will need Chlor and IAA chemical
• If you are using a DNA program and bacteria from the Blue-it kit, you will need Chlor and X-gal chemical
• If you are using a DNA program and bacteria from the Induce-it kit, you will need Amp and IPTG
• If you are using your own DNA program and bacteria, select accordingly


• Instructions: a manual with easy-to-understand vocabulary and step-by-step procedures and an online experiment simulation to practice with, teach you fundamentals, and guide you along the way. See the Resources section below for links

To complete this experiment, you will need to have access to either the DNA Playground or, if you have your own laboratory equipment, a 30 to 37°C incubator, a hot water bath with thermometer, crushed ice & ice bucket. You can also have a look at the instruction manual to learn how to make your own hot water bath/incubator. Note that your experiment result may vary based on how reliable you make your own equipment.

Cleaning and safety supplies are not included in the kit. Necessary safety and cleaning supplies are latex or nitrile gloves, and a chlorinated bleach cleaner or a 10% solution of chlorinated bleach in a spray bottle (you can make this by adding 9 parts water to 1 part bleach in a spray bottle). You can add a Mini Safety Set to your kit which includes gloves and a reusable plastic apron to protect your clothes.

For more information about safety, set up and clean up, you can have a look at the instruction manual for the experiment linked in the Ressources section below, and at the "Practicing safe science" page.

Shipping Times

Experiment kits are shipped 1-5 working days after you place your order. If you require yours for a specific date or event, please contact us before or with your order # so that we can try to make it happen for you! ☺︎ 

Storage

The experiment kit(s) must be refrigerated upon arrival for best shelf life. If your experiment kits stay at room temperature before you collect it from your mailbox/mailroom/etc, that's ok! As long as kits do not freeze, and they are stored in a refrigerator if you are not using it right away, there's no need to worry. If you do not have access to a refrigerator to store your kit, use it as soon as possible, and place it in a cool dark place until use.

For any questions, don't hesistate to reach out to us - help (at) amino.bio