Full STEAM ahead with the Yeast Engineer-it Kit - Metabolic Selection. Engineer yeast with DNA using metabolic selection to create a pink pigment!
This kit contains all ingredients needed to grow and engineer yeast with DNA to create pink fluorescent pigment.
Learn genetic engineering hands-on using a Yeast Engineer-it Kit. At the end of this life science project, you'll have grown and engineered yeast to produce something for you -- a colorful bio-pigment! A perfect way for beginners and experts alike to do fun and safe biotechnology experiments at home, in the classroom, the makerspace, community center, or in a local biohacking DIYbio lab!
Yeast can be engineered just as bacteria can using the antibiotic selection as you might have seen in the other Yeast Engineer-it Kit with Arctic Green DNA. As a more complex organism, yeast can also be engineered using a process called metabolic selection. In this Yeast Engineer-it Kit for Metabolic Selection, you will grow blank yeast and engineer-it using a DNA plasmid with a gene for creating a pink-colored pigment and a gene called URA3.
URA3 gives yeast the ability to synthesize (create) an enzyme called Orotidine 5'-phosphate decarboxylase (ODCase), so they can in turn create an essential nutrient for survival. In short, your blank yeast are unable to grow unless you either give them the essential nutrient they can not make, or enable them to produce the missing enzyme so they can create the nutrient themselves. This is a very different kind of selection!
With this kit, you will see how engineering different organisms can be done using similar processes using different principles.
If you haven't already, you may want to consider starting with the Yeast Canvas Kit, a fun biology-meets-art kit to learn how to grow yeast and make agar Petri dishes and the Yeast Engineer-it Kit in Arctic Green DNA. With these basic skills, you can move on to the Yeast Engineer-it Kit with confidence and complete your first genetic engineering experiment using metabolic selection!
Unsure what biotechnology and genetic engineering are? Have a look at our short "What is Biotechnology? Genetic Engineering?" post. And remember, no prior knowledge or skills are necessary to get started with Amino Labs beginner's biotechnology kits!
To complete this experiment, you will need to have access to either the DNA Playground or, if you have your own laboratory equipment, a 30 to 37°C incubator, a hot water bath with thermometer, crushed ice & ice bucket. You can also have a look at the instruction manual to learn how to make your own hot water bath/incubator. Note that your experiment result may vary based on how reliable you make your own equipment.
Cleaning and safety supplies are not included in the kit. Necessary safety and cleaning supplies are latex or nitrile gloves, and a chlorinated bleach cleaner or a 10% solution of chlorinated bleach in a spray bottle (you can make this by adding 9 parts water to 1 part bleach in a spray bottle). You can add a Mini Safety Set to your kit which includes gloves and a reusable plastic apron to protect your clothes.
For more information about safety, set up and clean up, you can have a look at the instruction manual for the experiment linked in the Ressources section below, and at the "Practicing safe science" page.
Experiment kits are shipped 1-5 working days after you place your order. If you require yours for a specific date or event, please contact us before or with your order # so that we can try to make it happen for you! ☺︎
The experiment kit(s) must be refrigerated upon arrival for best shelf life. If your experiment kits stay at room temperature before you collect it from your mailbox/mailroom/etc, that's ok! As long as kits do not freeze, and they are stored in a refrigerator if you are not using it right away, there's no need to worry. If you do not have access to a refrigerator to store your kit, use it as soon as possible, and place it in a cool dark place until use.
For any questions, don't hesistate to reach out to us - help (at) amino.bio