About
Overview
Based on the original genetic engineering Engineer-it Kit design, this STEM science project pack enables a junior researcher to complete DNA transformations with their own DNA programs and cells.
Have you purchased or acquired a DNA plasmid that you'd like to put into E. coli bacteria? Have you completed some ligations and want to transform E. coli with your ligated products? Then this kit pack is for you!
The Junior R&D Kit Pack comes with 8x separately packaged Junior 'Engineer-it' R&D kits so that you can try eight bacterial transformations with your own DNA plasmid in the same way you completed the Engineer-it Kit experience.
Select the type of antibiotic you need for your experiments from the drop-down, and get ready for transitioning to your own life science biotechnology R&D project.
If you're unsure of the antibiotic you need for your DNA plasmid and science project, message us using the chat app on the website or via email info@amino.bio.
With this kit, you will:
- Make selective & non-selective agar,
- pour Petri dishes,
- streak bacteria using your existing blank bacteria,
- make bacteria chemically competent,
- engineer bacteria using pigment-producing DNA plasmid (bacterial transformation) using your own DNA plasmid,
- incubate,
- view/analyze results
During this biotechnology genetic engineering experiment, you will refine your skills in genetic engineering, life science and learn how to re-use DNA programs to make with biology.
What's included & what's required
- (8) Junior R&D Kits: Sterile water, 6cm Petri dish, LB agar, selection marker, ‘blank’ bacteria stab, transformation buffer, recovery media, streaking stencils, inactivation bag. DNA plasmid not included - use your own DNA plasmid.
- Instructions: a manual with easy to understand vocabulary and step by step procedures and an online experiment simulation to practice with, teach you fundamentals, and guide you along the way. Available online. See the Resources tab for links!
The experiment kits must be refrigerated upon arrival for a longer shelf life.
Necessary equipment: Either the DNA Playground (home or large) or BioExplorer or 37 °C incubator + ice bucket + crushed ice + thermometer + hot water bath.
Cleaning and safety supplies are not included. These supplies are latex or nitrile gloves, a chlorinated cleaner, or a solution of chlorinated bleach in a spray bottle. For more information about safety, set up and clean up, have a look at the instruction manual(s) for your kit or mini lab.
Resources
- Try the Engineer-it kit Virtual Bioengineer Simulator.
- Browse the Engineer-it kit instructions
- Get the Zero to Genetic Engineering Hero book - Chapter 2 is about setting up your laboratory equipment, and space, and Chapter 4 is all about genetic engineering with the Engineer-it kit.
- Are you a teacher? We have lesson guides for setting up your laboratory space & biotechnology/life science safety, and for growing & discovering and engineering E. Coli cells using the Engineer-it kit in class!
- watch the real-time, step by step kit experience on Youtube.
- Try the course on Udemy to guide you through the Engineer-it Kit experience and provides confidence, context, and theory. Add it now for 25% off!
- Follow the real-time video tutorials on youtube!
Shipping Time
Kit Packs are shipped 1-5 working days after you place your order. If you require yours for a specific date or event, please contact us before or with your order # so that we can try to make it happen for you! ☺︎
The experiment kit(s) must be refrigerated upon arrival for a longer shelf life.