Engineer cells with a DNA program where gene expression can be manually controlled with temperature. (Manually control genes!)
Discover the Heat-it Kit, a biotechnology education kit that lets you grow and engineer bacteria with a DNA plasmid that can be controlled with heat. Decide when you want the DNA program to turn on by choosing the temperature at which you grow the bacteria. Turn the DNA program on and off to create tree ring patterns in the growth.
This kit is also a great addition to your BioArt toolkit!
This is an all-in-one STEM project kit with no prior experience necessary though prior completion of the Canvas kit and Engineer-it Kit can be helpful. A perfect step in your journey in exploring the world of biotechnology, biohacking & genetic engineering. Compatible with Ch. 7 of Zero to Genetic Engineering Hero book to learn what are genes, how to control them manually using genetic switches, and how to use them in biotechnology, synthetic biology, and for your science fair projects.
To complete this experiment, you will need to have access to either the DNA Playground or, if you have your own laboratory equipment, a 30 to 37°C incubator, a hot water bath with thermometer, crushed ice & ice bucket. You can also have a look at the instruction manual to learn how to make your own hot water bath/incubator. Note that your experiment result may vary based on how reliable you make your own equipment.
Cleaning and safety supplies are not included in the kit. Necessary safety and cleaning supplies are latex or nitrile gloves, and a chlorinated bleach cleaner or a 10% solution of chlorinated bleach in a spray bottle (you can make this by adding 9 parts water to 1 part bleach in a spray bottle). You can add a Mini Safety Set to your kit which includes gloves and a reusable plastic apron to protect your clothes.
For more information about safety, set up and clean up, you can have a look at the instruction manual for the experiment linked in the Ressources section below, and at the "Practicing safe science" page.
Experiment kits are shipped 1-5 working days after you place your order. If you require yours for a specific date or event, please contact us before or with your order # so that we can try to make it happen for you! ☺︎
The experiment kit(s) must be refrigerated upon arrival for best shelf life. If your experiment kits stay at room temperature before you collect it from your mailbox/mailroom/etc, that's ok! As long as kits do not freeze, and they are stored in a refrigerator if you are not using it right away, there's no need to worry. If you do not have access to a refrigerator to store your kit, use it as soon as possible, and place it in a cool dark place until use.
For any questions, don't hesistate to reach out to us - help (at) amino.bio