Cause a color-changing reaction to happen using an enzyme you extracted from cells that you engineered!
Discover the Blue-it Kit, a biotechnology education kit that lets you grow and engineer bacteria with a DNA program to create an enzyme that will cause a chemical reaction resulting in color-changing molecules. Extract your enzyme from the bacteria and mix it into specialized buffers to see different colors appear!
This is an all-in-one STEM project kit with no prior experience necessary though prior completion of the Canvas kit, Engineer-it Kit, and Extract-it kit can be helpful and is suggested. A perfect step in your journey in exploring the world of biotechnology, biohacking & genetic engineering. Compatible with Ch. 6 of Zero to Genetic Engineering Hero book to learn what is an enzyme, what are enzymatic reactions, and how to use them in biotechnology, synthetic biology, and for your science fair projects.
A microcentrifuge is required.
To complete this experiment, you will need to have access to either the DNA Playground and the Amino Labs Microcentrifuge or, if you have your own laboratory equipment, a 37°C incubator, a hot water bath with thermometer, crushed ice & ice bucket, and a microcentrifuge that spins at at least 13,000 x g.
Cleaning and safety supplies are not included in the kit. Necessary safety and cleaning supplies are latex or nitrile gloves, and a chlorinated bleach cleaner or a 10% solution of chlorinated bleach in a spray bottle (you can make this by adding 9 parts water to 1 part bleach in a spray bottle). You can add a Mini Safety Set to your kit which includes gloves and a reusable plastic apron to protect your clothes.
For more information about safety, set up and clean up, you can have a look at the instruction manual for the experiment linked in the Ressources section below, and at the "Practicing safe science" page.
Experiment kits are shipped 1-5 working days after you place your order. If you require yours for a specific date or event, please contact us before or with your order # so that we can try to make it happen for you! ☺︎
The experiment kit(s) must be refrigerated upon arrival for best shelf life, and the little bag(s) identified with a "Freezer bag" sticker must be placed in a freezer until use. Note that the larger, main kit bags should never be frozen - only the small bag with the "Freezer" sticker that accompanies it.
If your kits stay at room temperature before you collect it from your mailbox/mailroom/etc, that's ok! As long as the box does not freeze while left outside there's no need to worry (if it does, reach out to us for help).
Once you can, place the kit in the refrigerator and the small bag in the freezer. If you do not have access to a refrigerator/freezer to store your items, use them as soon as possible, and place it in a cool dark place until use.
For any questions, don't hesistate to reach out to us - help (at) amino.bio