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Blue-it kit enzymatic reactions - Learn genetic engineering skills while discovering biohacking, biotechnology, life science, DNA, what is an enzyme, enzymatic reactions with this STEM project kit, science kit, biohacking kit for home science, school science, makerspace, DIYbio, iGEM
Blue-it kit enzymatic reactions - Learn genetic engineering skills while discovering biohacking, biotechnology, life science, DNA, what is an enzyme, enzymatic reactions with this STEM project kit, science kit, biohacking kit for home science, school science, makerspace, DIYbio, iGEM
Blue-it kit enzymatic reactions - Learn genetic engineering skills while discovering biohacking, biotechnology, life science, DNA, what is an enzyme, enzymatic reactions with this STEM project kit, science kit, biohacking kit for home science, school science, makerspace, DIYbio, iGEM
Blue-it kit enzymatic reactions - Learn genetic engineering skills while discovering biohacking, biotechnology, life science, DNA, what is an enzyme, enzymatic reactions with this STEM project kit, science kit, biohacking kit for home science, school science, makerspace, DIYbio, iGEM
US$98.00

Blue-it Kit

Cause a color-changing reaction to happen using an enzyme you extracted from cells that you engineered!

Discover the Blue-it Kit, a biotechnology education kit that lets you grow and engineer bacteria with a DNA program to create an enzyme that will cause a chemical reaction resulting in color-changing molecules. Extract your enzyme from the bacteria and mix it into specialized buffers to see different colors appear!

This is an all-in-one STEM project kit with no prior experience necessary though prior completion of the Canvas kit, Engineer-it Kit and Extract-it kit can be helpful and is suggested. A perfect step in your journey exploring the world of biotechnology, biohacking & genetic engineering. Compatible with Ch. 6 of  Zero to Genetic Engineering Hero book to learn what is an enzyme, what are enzymatic reactions, and how to use them in biotechnology, synthetic biology, and for your science fair projects. 

A microcentrifuge is required.

  1. Make selective & non-selective agar,
  2. pour Petri dishes,
  3. streak bacteria,
  4. make bacteria chemically competent,
  5. engineer bacteria using DNA plasmid (bacterial transformation),
  6. create positive and negative controls,
  7. incubate,
  8. culture bacteria,
  9. lyse bacteria,
  10. filter-sterilize extract,
  11. add substrates to catalyze chemical reactions,
  12. see results.

During this biotechnology experiment, you will learn what an enzyme is and how to use them to make with biology. 

Learn genetic engineering skills while discovering biohacking, biotechnology, life science, DNA, what is an enzyme, enzymatic reactions with these STEM project science kit instructions.

Contents: 

  • (1) Blue-it Kit in Individual or Group size: Sterile water, 6cm Petri dish, LB agar, selection marker, ‘blank’ bacteria stab, transformation buffer, recovery media, positive control bacteria stab, DNA plasmid, lysis buffers, syringes, reaction buffer, stabilizer, streaking stencils, inactivation bag.
    • Individual Kits: An individual kit with all the ingredients needed for completing one entire science experience. Great for an individual or a small group.
    • Classroom/Group Kits: Four individually packaged kits with the needed ingredients to complete four entire science experiences. 1 shared resources bag containing the bacteria, DNA plasmid, and inactivation bags, which are shared between all participants. Great for the classroom, advanced AP biology, science fair projects, and iGEM teams.
  • Instructions: a manual with easy to understand vocabulary and step by step procedures and an online experiment simulation to practice with, teach you fundamentals, and guide you along the way. Available online. See the Resources tab for links!

The kit must be refrigerated upon arrival for longer shelf life. A small bag stapled to the outside of the kit and labeled "FREEZE" must be kept frozen until use. 

Necessary equipment: Microcentrifuge (13,000 x g minimum) and either the DNA Playground (home or large) or BioExplorer or 37 °C incubator + ice bucket + crushed ice + thermometer + hot water bath. 

Cleaning and safety supplies are not included. These supplies are latex or nitrile gloves, a chlorinated cleaner, or a solution of chlorinated bleach in a spray bottle. For more information about safety, set up and clean up, have a look at the instruction manual(s) for your kit or mini lab. 

  • Try the Engineer-it kit Virtual Bioengineer Simulator. - This is like the first part of the Blue-it kit experiment
  • Browse the Blue-it kit instructions
  • Get the Zero to Genetic Engineering Hero book - Chapter 2 is about setting up your laboratory equipment, and space. Chapter 6 is all about genetic engineering and enzymatic reactions with the Blue-it kit.
  • Are you a teacher? We have lesson guides for setting up your laboratory space & biotechnology/life science safety, and for growing & discovering and engineering E. Coli cells using the Engineer-it kit in class!

Experiment kit(s) ship in 1-10 working days. If you require yours for a specific date or event, please contact us before or with your order # so that we can make it happen for you! ☺︎ 

The experiment kit(s) must be refrigerated upon arrival for a longer shelf life. A small bag stapled to the outside of the kit and labeled "FREEZE" must be kept frozen until use. 

 

Overview

Cause a color-changing reaction to happen using an enzyme you extracted from cells that you engineered!

Discover the Blue-it Kit, a biotechnology education kit that lets you grow and engineer bacteria with a DNA program to create an enzyme that will cause a chemical reaction resulting in color-changing molecules. Extract your enzyme from the bacteria and mix it into specialized buffers to see different colors appear!

This is an all-in-one STEM project kit with no prior experience necessary though prior completion of the Canvas kit, Engineer-it Kit and Extract-it kit can be helpful and is suggested. A perfect step in your journey exploring the world of biotechnology, biohacking & genetic engineering. Compatible with Ch. 6 of  Zero to Genetic Engineering Hero book to learn what is an enzyme, what are enzymatic reactions, and how to use them in biotechnology, synthetic biology, and for your science fair projects. 

A microcentrifuge is required.

In this kit, you will:

  1. Make selective & non-selective agar,
  2. pour Petri dishes,
  3. streak bacteria,
  4. make bacteria chemically competent,
  5. engineer bacteria using DNA plasmid (bacterial transformation),
  6. create positive and negative controls,
  7. incubate,
  8. culture bacteria,
  9. lyse bacteria,
  10. filter-sterilize extract,
  11. add substrates to catalyze chemical reactions,
  12. see results.

During this biotechnology experiment, you will learn what an enzyme is and how to use them to make with biology. 

Learn genetic engineering skills while discovering biohacking, biotechnology, life science, DNA, what is an enzyme, enzymatic reactions with these STEM project science kit instructions.

What's Included & what's required

Contents: 

  • (1) Blue-it Kit in Individual or Group size: Sterile water, 6cm Petri dish, LB agar, selection marker, ‘blank’ bacteria stab, transformation buffer, recovery media, positive control bacteria stab, DNA plasmid, lysis buffers, syringes, reaction buffer, stabilizer, streaking stencils, inactivation bag.
    • Individual Kits: An individual kit with all the ingredients needed for completing one entire science experience. Great for an individual or a small group.
    • Classroom/Group Kits: Four individually packaged kits with the needed ingredients to complete four entire science experiences. 1 shared resources bag containing the bacteria, DNA plasmid, and inactivation bags, which are shared between all participants. Great for the classroom, advanced AP biology, science fair projects, and iGEM teams.
  • Instructions: a manual with easy to understand vocabulary and step by step procedures and an online experiment simulation to practice with, teach you fundamentals, and guide you along the way. Available online. See the Resources tab for links!

The kit must be refrigerated upon arrival for longer shelf life. A small bag stapled to the outside of the kit and labeled "FREEZE" must be kept frozen until use. 

Necessary equipment: Microcentrifuge (13,000 x g minimum) and either the DNA Playground (home or large) or BioExplorer or 37 °C incubator + ice bucket + crushed ice + thermometer + hot water bath. 

Cleaning and safety supplies are not included. These supplies are latex or nitrile gloves, a chlorinated cleaner, or a solution of chlorinated bleach in a spray bottle. For more information about safety, set up and clean up, have a look at the instruction manual(s) for your kit or mini lab. 

Resources

  • Try the Engineer-it kit Virtual Bioengineer Simulator. - This is like the first part of the Blue-it kit experiment
  • Browse the Blue-it kit instructions
  • Get the Zero to Genetic Engineering Hero book - Chapter 2 is about setting up your laboratory equipment, and space. Chapter 6 is all about genetic engineering and enzymatic reactions with the Blue-it kit.
  • Are you a teacher? We have lesson guides for setting up your laboratory space & biotechnology/life science safety, and for growing & discovering and engineering E. Coli cells using the Engineer-it kit in class!

Shipping Times

Experiment kit(s) ship in 1-10 working days. If you require yours for a specific date or event, please contact us before or with your order # so that we can make it happen for you! ☺︎ 

The experiment kit(s) must be refrigerated upon arrival for a longer shelf life. A small bag stapled to the outside of the kit and labeled "FREEZE" must be kept frozen until use.