Get ready to uncover the secrets of DNA with Gel Electrophoresis Kit: Learning DNA Ladders! Run mixtures of fragments of different sizes and compare them to a DNA ladder. It's a hands-on way to visualize and understand the sizes of DNA fragments.
With this kit, you will run a mixture of fragments of various sizes and a DNA Ladder in a gel so that you can identify the fragment sizes. We recommend this kit as a follow-up to the Gel Electrophoresis Kit: Natural vs Synthetic Dyes and the Gel Electrophoresis Kit: Draw with DNA. Once you have successfully undertaken a DNA gel electrophoresis experiment that resulted in some DNA Gel Art, you will move onto the type of experiment professional scientists might use in a lab to learn more about DNA: Using a DNA Ladder to estimate the size of experimental DNA fragments.
Infact, the method you’re about to undertake is used in disciplines such as forensics to analyze DNA fingerprints that are generated using polymerase chain reaction of crime scene DNA. Quite often it is used in genetic engineering experiments to assess the size of DNA fragments that one wants to insert into other pieces of DNA - something you’ll experience in the coming DNA Technology experiments.
From a hands-on skills standpoint, you will:
- Practice casting an agarose gel
- Practice loading DNA into gel wells
- You will add a DNA ladder into a gel well
- Practice running the gel
What will you learn?
What else is needed?
Cleaning and safety supplies are not included in the kit. Necessary safety and cleaning supplies are latex or nitrile gloves, and a chlorinated bleach cleaner or a 10% solution of chlorinated bleach in a spray bottle (you can make this by adding 9 parts water to 1 part bleach in a spray bottle). You can add a Mini Safety Set to your kit which includes gloves and a reusable plastic apron to protect your clothes.
For more information about safety, set up and clean up, you can have a look at the instruction manual for the experiment linked in the Ressources section below, and at the "Practicing safe science" page.
Storage & Shipping
Experiment kits are shipped 1-10 working days after you place your order. If you require yours for a specific date or event, please contact us before or with your order # so that we can try to make it happen for you! ☺︎
For any questions, don't hesistate to reach out to us - contact us