Induce-it Kit™: Control gene expression using chemicals

How can you decide when your engineered bacteria activate? With the Induce-it Kit, you can manually control gene expression!

Discover the Induce-it Kit, a biology education kit that lets you practice inducing (turning on) gene expression with a chemical. Create petri dishes, streak, incubate, and perform a bacterial transformation using the DNA included in the kit. Once your cells are ready, amplify your culture and add your IPTG inducer! Choose which cells you want to ‘turn on’ by adding the inducer chemical only to certain area(s) of your petri dish. 

This is an all-in-one STEM project kit with no prior experience required, though completing the Canvas kit and the Engineer-it Kit can be helpful and is suggested. A perfect step in your journey exploring the world of biotechnology, biohacking & genetic engineering. Compatible with Ch. 7 of the Zero to Genetic Engineering Hero book to learn what genes are, how to control them manually using genetic switches, and how to use them in biotechnology, synthetic biology, and for your science fair projects.

During this biotechnology experiment, you will learn how to control genes with heat and how to use that to make with biology.

Specifically, with this kit, you will:
1. Make selective & non-selective agar,
2. pour Petri dishes,
3. streak bacteria,
4. make bacteria chemically competent,
5. engineer bacteria using DNA plasmid (bacterial transformation),
6. create positive and negative controls,
7. incubate,
8. culture bacteria,
9. add inducer to activate the genetic switches
10. see results.

(1) Induce-it Kit in Individual or Group size: Sterile water, 6cm Petri dish, LB agar, selection marker, ‘blank’ bacteria stab, transformation buffer, recovery media, positive control bacteria stab, DNA plasmid, inducer, drop pipet, dissolving buffer, streaking stencils, inactivation bag.

• Individual Kits: An individual kit with all the ingredients needed for completing one entire science experience. Great for an individual or a small group.

• Classroom/Group Kits: Four individually packaged kits with the needed ingredients to complete four entire science experiences. 1 shared resources bag containing the bacteria, DNA plasmid, and inactivation bags, which are shared between all participants. Great for the classroom, advanced AP biology, science fair projects, and iGEM teams.

• Instructions: a manual with easy-to-understand vocabulary and step-by-step procedures and an online experiment simulation to practice with, teach you fundamentals, and guide you along the way. Available online. See the Resources tab for links!

To complete this experiment, you will need to have access to either the DNA Playground or, if you have your own laboratory equipment, a 37°C incubator, a hot water bath, crushed ice & ice bucket.

Cleaning and safety supplies are not included in the kit. Necessary safety and cleaning supplies are latex or nitrile gloves, and a chlorinated bleach cleaner or a 10% solution of chlorinated bleach in a spray bottle (you can make this by adding 9 parts water to 1 part bleach in a spray bottle). You can add a Mini Safety Set to your kit which includes gloves and a reusable plastic apron to protect your clothes.

For more information about safety, set up and clean up, you can have a look at the instruction manual for the experiment linked in the Ressources section below, and at the "Practicing safe science" page.

Shipping Times

Experiment kits are shipped 1-10 working days after you place your order. If you require yours for a specific date or event, please contact us before or with your order # so that we can try to make it happen for you! ☺︎ 

Storage

The experiment kit(s) must be refrigerated upon arrival for the best shelf life, and the little bag(s) identified with a "Freezer bag" sticker must be placed in a freezer until use. Note that the main kit bags should never be frozen - only freeze the small bag with the "Freezer" sticker.

If your kits stay at room temperature before you collect it from your mailbox/mailroom/etc, that's ok! Place the kit(s) in the refrigerator and the small bag in the freezer as soon as you can. If you do not have access to a refrigerator/freezer for storage, use as soon as possible, and place it in a cool dark place until use.

For any questions, don't hesistate to reach out to us - contact us