About the Zero to Genetic Engineering Hero™: 5 - 7 (Home Pack)
Building off the knowledge and skills gained in the Zero to Genetic Engineering Hero Pack (Chapters 1-4), this new biotechnology kit pack includes a series of advanced kits to push your learning further. Learners will continue their journey towards more advanced subjects like extracting proteins from the cells they have engineered, expressing enzymes to catalyze chemical reactions, and controlling the expression of genes using chemicals, and heat.
The life science experiment kits included in this pack will take you through the second part of the Genetic Engineering Hero's journey. In addition to completing the engaging hands-on activities mentioned above, you will also gain and practice further real-world lab skills such as microcentrifugation, culturing cells, inducing expression, and filter sterilization. While the hands-on activities and learnings in Chapters 5-7 delve into more advanced genetic engineering concepts and skills, you do not need any prior knowledge to do them. You only need to have completed Chapters 1 through 4 of the Zero to Genetic Engineering Hero Journey.
This kit pack includes individual experiment kits and is ideal for individuals, small groups, and families to extend their knowledge in the exciting and promising world of biotechnology.
“The book, the DNA Playground, Zero to Hero starter kits, and my centrifuge gave me an understanding of what genetic engineering is and how you can program bacteria to make things. It was so exciting that I’m asking my science teacher to start an iGEM team next year that will compete in the High School iGEM league.” Pau, 12-year-old, Washington, DC, USA.
Patricia, a 14-year-old from Markham, Canada, started her Zero to Genetic Engineering Hero journey at age 12 and was recently featured at MIT Media Lab's global biosummit, and on the news for her award-winning science projects. Hear Patricia talk about her work to CBC News here.
What will you learn?
Chapter 5 - Extracting your engineered proteins
- extract the proteins created by your engineer bacteria in real-life using the Extract-it kit so that they can be used in the 'real-world' (optional hands-on),
- continue understanding how cells know how to start, do and stop reading RNA (translation),
- discover proteins & enzymes, promoters vs. the ribosomal binding site and the other RNAs (rRNA & tRNA),
- learn and use the RNA to protein cipher.
Chapter 6 - Processing Chemicals with Enzymes
- engineer cells to created small molecules and enzymes to create products like smells and color-changing molecules using the Smell-it kit and the Blue-it kit (optional hands-on),
- learn the basics of enzymatic chemical reactions,
- understand the Four B’s of cell function in action,
- discover protein enzymes as catalysis in cells,
- learn the basic structure of atoms and the four major types of bonds,
- explorer metabolic pathways and how they are used in genetic engineering and biotechnology.
Chapter 7 - Manually turning on genes in situ
- engineer cells or use pre-engineered cells and manually turn on genes using light, chemicals, and heat to understand genetic switched in real-life using the RGB kit, the Induce-it kit and the Heat-it kit (optional hands-on),
- learn the mechanisms of gene expression,
- revise and apply the coding and non-coding regions of genes knowledge from Ch. 4-5,
- explore a DNA plasmid map,
- start setting the stage for you to design your custom DNA plasmids.
See the full learning journey map here
What else is needed?
Cleaning and safety supplies are not included in the kit. Necessary safety and cleaning supplies are latex or nitrile gloves, and a chlorinated bleach cleaner or a 10% solution of chlorinated bleach in a spray bottle (you can make this by adding 9 parts water to 1 part bleach in a spray bottle). You can add a Mini Safety Set to your kit which includes gloves and a reusable plastic apron to protect your clothes.
For more information about safety, set up and clean up, you can have a look at the instruction manual for the experiment linked in the Ressources section below, and at the "Practicing safe science" page.
Storage & Shipping
Experiment kits are shipped 1-10 working days after you place your order. If you require yours for a specific date or event, please contact us before or with your order # so that we can try to make it happen for you! ☺︎
For any questions, don't hesistate to reach out to us - contact us