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Amino Labs' Zero to Genetic Engineering hero science classroom starter pack -  Learn what is DNA, what is a gene, cell theory, genetic engineering, enzymatic reaction, protein extractions, biotechnology, biohacking and genetic engineering with the world's first biohacking and biotechnology STEM beginner's starter kits
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glowing bacteria on petri dish with heat activated genetic switch made with Amino Labs’ Zero to Genetic Engineering hero starter pack for  middle school science, biology, genetics, high school science, biology, genetics, NGSS.  Learn what is DNA, what is a gene, cell theory, genetic engineering, bioart, biotechnology, biohacking and genetic engineering with the world's first biohacking and biotechnology STEM beginner's book and starter pack of science project kits, biotechnology project kits
glowing bacteria on petri dish with heat activated genetic switch made with Amino Labs’ Zero to Genetic Engineering hero starter pack for  middle school science, biology, genetics, high school science, biology, genetics, NGSS.  Learn what is DNA, what is a gene, cell theory, genetic engineering, bioart, biotechnology, biohacking and genetic engineering with the world's first biohacking and biotechnology STEM beginner's book and starter pack of science project kits, biotechnology project kits
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Microcentrifuge for biohacking beginners, genetic engineering beginners, DIYbio, life science class, AP biology class laboratory equipment.
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US$2,131.00

Zero to Genetic Engineering Hero Classroom Pack (Chapters 5-7)

Building off of Zero to Genetic Engineering Hero Classroom Pack (Chapters 1-4), this new biotechnology kit pack includes a series of advanced kits that leverage the skills and knowledge learned in previous chapters. Students will continue towards more advanced subjects such as extracting proteins from the cells they have engineered, expressing enzymes to catalyze chemical reactions, and controlling the expression of genes using chemicals, heat, and light. 

The life science experiment kits included in this pack will take learners through the second part of the Genetic Engineering Hero's journey. In addition to completing the engaging hands-on activities mentioned above, students will also gain and practice further real-world lab skills such as microcentrifugation, culturing cells, inducing expression, and filter sterilization. While the hands-on activities and learnings in Chapters 5-7 delve into more advanced genetic engineering, these experiments can be used by teachers and students with no life science research experience. No prior knowledge is necessary save for having had completed Chapters 1 through 4 of the Zero to Genetic Engineering Hero Journey.

This Classroom Pack has four (4) of each kit for the exercises in Chapters 5-7 of Zero to Genetic Engineering Hero to extend students' knowledge in the exciting and promising world of biotechnology. 

 

discover amino labs success guarantee for all home science experiment kits in biotechnology, diy bio

Over the course of 31* hands-on periods**, your students will:

  • Chapter 5 - Extracting your engineered proteins
    • learn how to extract the proteins created by your engineer bacteria in real-life using the Extract-it kit,
    • continue understanding how cells know how to start, do and stop reading RNA (translation),
    • discover proteins & enzymes, promoters vs. the ribosomal binding site and the other RNAs (rRNA & tRNA),
    • learn and use the RNA to protein cipher.
  • Chapter 6 - Processing Chemicals with Enzymes
    • learn how to engineer cells to created small molecules and enzymes to create products like smells and color-changing molecules using the Smell-it kit and the Blue-it kit,
    • learn the basics of enzymatic chemical reactions,
    • understand the Four B’s of cell function in action,
    • discover protein enzymes as catalysis in cells,
    • learn the basic structure of atoms and the four major types of bonds,
    • explorer metabolic pathways and how they are used in genetic engineering and biotechnology. 
  • Chapter 7 - Manually turning on genes in situ
    • learn how to engineer cells or use pre-engineered cells and manually turn on genes using light, chemicals, and heat. Understand genetic switched in real-life using the RGB kit, the Induce-it kit, and the Heat-it kit,
    • learn the mechanisms of gene expression,
    • revise and apply the coding and non-coding regions of genes knowledge from Ch. 4-5,
    • explore a DNA plasmid map,
    • start setting the stage for you to design your custom DNA plasmids.

During these STEM biotechnology experiments, students will continue learning how to leverage bacteria, genes, chemistry, and DNA to make with biology.

After completing these hands-on activities, students will know how to extract products from cells, use genetic engineering to cause enzymatic reactions, and how to control and activate genes manually.

View the full learning journey here.

*Hands-on experiences from the same chapters can be run concurrently to cut down on the number of periods required to complete this journey, cutting down the total hands-on class time to 16 periods.

**This timeline is restricted to hands-on activities. The time to teach the fundamental concepts can vary depending on your class' existing knowledge, how in-depth you want to explore each topic, and how involved your in-class discussions get!

  • (1) Extract-it Kit - Group - (Chapter 5)

The natural next step after a student learns how to engineer cells with a plasmid and how the cell reads the DNA plasmid gene (transcription) is to learn how to extract those engineered proteins from the cells. From a Fundamental concepts standpoint, the next step is to learn the process that the cells use to read the RNA and translate proteins (translation). The hands-on exercise of extracting and then holding in their hands the proteins the students have engineered is an excellent anchor point for the Fundamentals section of Chapter 5, Translation. In the hands-on exercise, the students use every skill they have learned in Chapters 1-4, including lysing cells, streaking cells, incubating cells, and engineering cells!

Because the advanced kits are usually completed in higher-level classes with fewer students, they come in packs of four kits. The Extract-it Kit comes with four individually packed kits that can be passed out to student groups. They require the use of the engineered cells that your students would have recently created from the Engineer-it Kit in Chapter 4. In addition to a DNA Playground Large, this kit requires the use of a microcentrifuge.

 

Smell-it Group Kit & Blue-it Kit (Chapter 6)

Now that the students have mastered the basic concepts of cell function and lab skills of genetic engineering, Chapter 6 dives deeper into the chemistry that makes cells function. It also addresses another critical topic of genetic engineering - using enzymes to cause chemical reactions. The Smell-it Kit enables students to first engineer bacteria with a plasmid that creates an enzyme and then culture those cells with a substrate added to the LB agar plates. The students marvel as their engineered cells transform the musky smelling substrate into an overripe banana scent. The Blue-it Kit also includes the engineering of bacteria to produce an enzyme. In the Blue-it Kit, however, the students engineer the cells, extract the engineered enzyme, and complete a chemical reaction in a tube. The enzyme will convert a white powder into a vivid blue chemical.

The Fundamentals of Chapter 6 use the hands-on exercises as anchor points and dive deeper into chemical reactions. An essential part of chemical reactions is bonding, and so a light introduction to atoms and the four types of bonds are discussed. Ultimately the students leave with an understanding of not only what proteins are but how they function.

Four Smell-it Kit and four Blue-it Kit are included as individually packed kits that can be handed out to students. These kits require that 4 Petri dishes per kit be simultaneously incubated, meaning that one DNA Playground Large can only be used by two groups of students at a time. In addition to a DNA Playground Large, the Blue-it Kit requires the use of a microcentrifuge.

 

Heat-it Kit, Induce-it Kit, and RGB Kit (Chapter 7)

With a good understanding of transcription (Chapter 4) and translation (Chapter 5) and the basic bonding used in cells (Chapter 6), students can move onto gene regulation. These three kits use three different environmental factors to control the expression of genes.

The Heat-it Kit includes a DNA plasmid with a gene that has a promoter that is responsive to temperature. At 30 °C the promoter is in an OFF state; however, at 37 °C and especially 42 °C, the promoter can activate the expression of a colored protein.  The Fundamentals section describes how the temperature-sensitive promoter relates to the cells' basic stress response, and in a sense, “hi-jacks” that response.

The Induce-it Kit explores the world of using chemicals to manipulate gene expression. In this instance, the students first engineer cells with a DNA plasmid containing a color-expressing gene that is sensitive to the presence of a chemical. After culturing their engineered cells, the students add the chemical to spots on the Petri dish and over a few hours, begin to see the expression of the color protein.

The RGB Kit includes the use of a strain of bacteria that is sensitive to light. Using the DNA Playground Large, which has an LED light in its incubator, the student chooses a color of light using the touchscreen, which causes the expression of different color pigments. Red LED light causes the cells to create red pigment. Green LED light causes the cells to make green pigment. Blue light causes the cells to produce blue pigment. In the Fundamentals section, the gene regulation relating to the green pigment is described in detail.

Four of each of the Heat-it Kit, Induce-it Kit, and RGB Kit are included as individually packaged units that can be handed out to students. 

  • Instructions: a manual for each kit, with easy to understand vocabulary and step by step procedures. Available online. See the Resources tab for links.

 

Optional, choose from the add-on: 

  • Microcentrifuge

A microcentrifuge is an essential piece of equipment that is used in every life science research laboratory in the world. A microcentrifuge is a small toaster-sized device that can spin tubes (1.5 mL lab tubes) at very high speeds. This process allows you to separate different components in your tubes and is an important part of doing science. The microcentrifuge offered as an optional add-on can hold up to 12 microcentrifuge tubes at a time and spin at 15,000 RPM (~14,000 x g). It has a timer, so you set a time, start the centrifuge, and with the timer reaches zero, it will automatically stop spinning.

If you already have a microcentrifuge, select "0" microcentrifuges in the dropdown.  

Zero to Genetic Engineering Hero Packs are shipped 1-5 working days after you place your order. However, the microcentrifuge(s) may be shipped separately and can take up to 3 weeks for fulfillment and may be shipped separatelyIf you require yours for a specific date or event, please contact us before or with your order # so that we can try to make it happen for you! ☺︎  

The experiment kit(s) must be refrigerated upon arrival for a longer shelf life. 

Some components also require freezing upon arrival until use. These will be placed in bags labeled 'FREEZE' stapled to the outside of the Blue-it Kit, Induce-it Kit, and the Plate Extract-it kit.

Overview

Building off of Zero to Genetic Engineering Hero Classroom Pack (Chapters 1-4), this new biotechnology kit pack includes a series of advanced kits that leverage the skills and knowledge learned in previous chapters. Students will continue towards more advanced subjects such as extracting proteins from the cells they have engineered, expressing enzymes to catalyze chemical reactions, and controlling the expression of genes using chemicals, heat, and light. 

The life science experiment kits included in this pack will take learners through the second part of the Genetic Engineering Hero's journey. In addition to completing the engaging hands-on activities mentioned above, students will also gain and practice further real-world lab skills such as microcentrifugation, culturing cells, inducing expression, and filter sterilization. While the hands-on activities and learnings in Chapters 5-7 delve into more advanced genetic engineering, these experiments can be used by teachers and students with no life science research experience. No prior knowledge is necessary save for having had completed Chapters 1 through 4 of the Zero to Genetic Engineering Hero Journey.

This Classroom Pack has four (4) of each kit for the exercises in Chapters 5-7 of Zero to Genetic Engineering Hero to extend students' knowledge in the exciting and promising world of biotechnology. 

 

discover amino labs success guarantee for all home science experiment kits in biotechnology, diy bio

In this pack, students will

Over the course of 31* hands-on periods**, your students will:

  • Chapter 5 - Extracting your engineered proteins
    • learn how to extract the proteins created by your engineer bacteria in real-life using the Extract-it kit,
    • continue understanding how cells know how to start, do and stop reading RNA (translation),
    • discover proteins & enzymes, promoters vs. the ribosomal binding site and the other RNAs (rRNA & tRNA),
    • learn and use the RNA to protein cipher.
  • Chapter 6 - Processing Chemicals with Enzymes
    • learn how to engineer cells to created small molecules and enzymes to create products like smells and color-changing molecules using the Smell-it kit and the Blue-it kit,
    • learn the basics of enzymatic chemical reactions,
    • understand the Four B’s of cell function in action,
    • discover protein enzymes as catalysis in cells,
    • learn the basic structure of atoms and the four major types of bonds,
    • explorer metabolic pathways and how they are used in genetic engineering and biotechnology. 
  • Chapter 7 - Manually turning on genes in situ
    • learn how to engineer cells or use pre-engineered cells and manually turn on genes using light, chemicals, and heat. Understand genetic switched in real-life using the RGB kit, the Induce-it kit, and the Heat-it kit,
    • learn the mechanisms of gene expression,
    • revise and apply the coding and non-coding regions of genes knowledge from Ch. 4-5,
    • explore a DNA plasmid map,
    • start setting the stage for you to design your custom DNA plasmids.

During these STEM biotechnology experiments, students will continue learning how to leverage bacteria, genes, chemistry, and DNA to make with biology.

After completing these hands-on activities, students will know how to extract products from cells, use genetic engineering to cause enzymatic reactions, and how to control and activate genes manually.

View the full learning journey here.

*Hands-on experiences from the same chapters can be run concurrently to cut down on the number of periods required to complete this journey, cutting down the total hands-on class time to 16 periods.

**This timeline is restricted to hands-on activities. The time to teach the fundamental concepts can vary depending on your class' existing knowledge, how in-depth you want to explore each topic, and how involved your in-class discussions get!

What's Included & what's required

  • (1) Extract-it Kit - Group - (Chapter 5)

The natural next step after a student learns how to engineer cells with a plasmid and how the cell reads the DNA plasmid gene (transcription) is to learn how to extract those engineered proteins from the cells. From a Fundamental concepts standpoint, the next step is to learn the process that the cells use to read the RNA and translate proteins (translation). The hands-on exercise of extracting and then holding in their hands the proteins the students have engineered is an excellent anchor point for the Fundamentals section of Chapter 5, Translation. In the hands-on exercise, the students use every skill they have learned in Chapters 1-4, including lysing cells, streaking cells, incubating cells, and engineering cells!

Because the advanced kits are usually completed in higher-level classes with fewer students, they come in packs of four kits. The Extract-it Kit comes with four individually packed kits that can be passed out to student groups. They require the use of the engineered cells that your students would have recently created from the Engineer-it Kit in Chapter 4. In addition to a DNA Playground Large, this kit requires the use of a microcentrifuge.

 

Smell-it Group Kit & Blue-it Kit (Chapter 6)

Now that the students have mastered the basic concepts of cell function and lab skills of genetic engineering, Chapter 6 dives deeper into the chemistry that makes cells function. It also addresses another critical topic of genetic engineering - using enzymes to cause chemical reactions. The Smell-it Kit enables students to first engineer bacteria with a plasmid that creates an enzyme and then culture those cells with a substrate added to the LB agar plates. The students marvel as their engineered cells transform the musky smelling substrate into an overripe banana scent. The Blue-it Kit also includes the engineering of bacteria to produce an enzyme. In the Blue-it Kit, however, the students engineer the cells, extract the engineered enzyme, and complete a chemical reaction in a tube. The enzyme will convert a white powder into a vivid blue chemical.

The Fundamentals of Chapter 6 use the hands-on exercises as anchor points and dive deeper into chemical reactions. An essential part of chemical reactions is bonding, and so a light introduction to atoms and the four types of bonds are discussed. Ultimately the students leave with an understanding of not only what proteins are but how they function.

Four Smell-it Kit and four Blue-it Kit are included as individually packed kits that can be handed out to students. These kits require that 4 Petri dishes per kit be simultaneously incubated, meaning that one DNA Playground Large can only be used by two groups of students at a time. In addition to a DNA Playground Large, the Blue-it Kit requires the use of a microcentrifuge.

 

Heat-it Kit, Induce-it Kit, and RGB Kit (Chapter 7)

With a good understanding of transcription (Chapter 4) and translation (Chapter 5) and the basic bonding used in cells (Chapter 6), students can move onto gene regulation. These three kits use three different environmental factors to control the expression of genes.

The Heat-it Kit includes a DNA plasmid with a gene that has a promoter that is responsive to temperature. At 30 °C the promoter is in an OFF state; however, at 37 °C and especially 42 °C, the promoter can activate the expression of a colored protein.  The Fundamentals section describes how the temperature-sensitive promoter relates to the cells' basic stress response, and in a sense, “hi-jacks” that response.

The Induce-it Kit explores the world of using chemicals to manipulate gene expression. In this instance, the students first engineer cells with a DNA plasmid containing a color-expressing gene that is sensitive to the presence of a chemical. After culturing their engineered cells, the students add the chemical to spots on the Petri dish and over a few hours, begin to see the expression of the color protein.

The RGB Kit includes the use of a strain of bacteria that is sensitive to light. Using the DNA Playground Large, which has an LED light in its incubator, the student chooses a color of light using the touchscreen, which causes the expression of different color pigments. Red LED light causes the cells to create red pigment. Green LED light causes the cells to make green pigment. Blue light causes the cells to produce blue pigment. In the Fundamentals section, the gene regulation relating to the green pigment is described in detail.

Four of each of the Heat-it Kit, Induce-it Kit, and RGB Kit are included as individually packaged units that can be handed out to students. 

  • Instructions: a manual for each kit, with easy to understand vocabulary and step by step procedures. Available online. See the Resources tab for links.

 

Optional, choose from the add-on: 

  • Microcentrifuge

A microcentrifuge is an essential piece of equipment that is used in every life science research laboratory in the world. A microcentrifuge is a small toaster-sized device that can spin tubes (1.5 mL lab tubes) at very high speeds. This process allows you to separate different components in your tubes and is an important part of doing science. The microcentrifuge offered as an optional add-on can hold up to 12 microcentrifuge tubes at a time and spin at 15,000 RPM (~14,000 x g). It has a timer, so you set a time, start the centrifuge, and with the timer reaches zero, it will automatically stop spinning.

If you already have a microcentrifuge, select "0" microcentrifuges in the dropdown.  

Resources

Shipping Times

Zero to Genetic Engineering Hero Packs are shipped 1-5 working days after you place your order. However, the microcentrifuge(s) may be shipped separately and can take up to 3 weeks for fulfillment and may be shipped separatelyIf you require yours for a specific date or event, please contact us before or with your order # so that we can try to make it happen for you! ☺︎  

The experiment kit(s) must be refrigerated upon arrival for a longer shelf life. 

Some components also require freezing upon arrival until use. These will be placed in bags labeled 'FREEZE' stapled to the outside of the Blue-it Kit, Induce-it Kit, and the Plate Extract-it kit.